Journal: Frontiers in Immunology
Article Title: Examining the Effect of Kindlin-3 Binding Site Mutation on LFA-1-ICAM-1 Bonds by Force Measuring Optical Tweezers
doi: 10.3389/fimmu.2021.792813
Figure Lengend Snippet: Force measuring optical tweezer system used for static adhesion assays. OBJ 1, Nikon 1.25 NA 100x oil immersion objective; OBJ 2, Mitutoyo 0.55NA 100x long working distance objective. DIC, dichroic mirror; QPD, quadrant photodiode; L, lens; M, mirror. Application-specific optics refer to expansion and beam steering optics. The sample was placed on a Thorlabs MAX302/M NanoMax piezoelectric stage, which was connected to a Thorlabs MDT630A 3-Axis piezo controller.
Article Snippet: Samples were placed on a Thorlabs MAX302/M NanoMax piezoelectric stage (Thorlabs, Newton, NJ, USA), which was connected to a Thorlabs MDT630A 3-Axis piezo controller.
Techniques: